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PIPELINE

Pipeline

NK cells (Natural Killer Cells) are the immune cells responsible for the primary immunity in human body.It is a fourth-generation anti-cancer treatment agent that can overcome the limitations of immune checkpoint inhibitors, and the research and development about these cells are being actively conducted worldwide.Our DR-NK cells are the only artificial NK cells developed through the direct differentiation from blood cells, rather than from natural NK cells or NK cells derived from IPSCs.

(Licensed from Dr Yeesuk Cho, KRIBB in 2020)

  • Artificially Induced NK

    · This is a natural killer cell (NK Cell) developed from the direct differentiation method that uses Yamanaka Factor but does not go through the IPS step from blood (PBMC).

    · Thisuses the world's only NK cell differentiation method that shortens the differentiation period, so improves the productivity and economic efficiency.

  • Direct differentiation technologyfor NK cell

    Direct differentiation technologyfor NK cell

Dual functions of proliferation/immune regulation (cytokine secretion) & killing activity

General NK cells have the ability to regulate the immune and inflammatory responses, but their ability to directly attack the cancer cells is poor.Therefore, the separate killing ability activation step is required, but it has the characteristic that when the activation function increases, the immune control ability decreases again.However, it can be confirmed that CEFObio's NK cells are CD56bright/CD16+ NK cells.This means that when administered in vivo, it has the ability to kill and at the same time induces the participation of other immune cells in the immune response through immune regulation (dual function).

  • Blood NK Cell and NK of other companies

    Blood NK Cell and NK of other companies
    Blood NK Cell and NK of other companies

  • DR-NK

    DR-NK
    DR-NK

  • Killing activity

    Killing activity

    · Killing ability was verified using NK92 cells as a control.

    · NK92 cells show about 30% killing ability even at 5:1

    · On the other hand, in the case of CEFObio NK cells, the killing ability is close to 95% at 5:1.

  • Cytokine Secretion

    Cytokine Secretion

    · The level of IFN-g secretion was checked to verify the immune modulating ability.

    · While NK92 cells increased approximately 2-fold compared to before stimulation,
    CEFObio NK cells increased 200-fold.

    *E:T ratio is the ratio of target cells (cancer cells) to effectors (NK cells).
    The experiment was conducted by increasing the ratio of NK cells up to 0.25, 1, 2.5, and 5 folds to 1 cancer cell.

As a result of confirming the tumor growth inhibition ability of NK cells in theMouse solid cancer model using the human colon cancer cells, similar results to the first-generation anticancer drug (Doxorubicin) were obtained at high concentrations.

  • Solid cancer In Vivo Test (Mouse Subcutaneous Tumor Transplantation Model) Cell line : SW620 (Human Caucasian colon adenocarcinoma)

    Solid cancer In Vivo Test (Mouse Subcutaneous Tumor Transplantation Model) Cell line : SW620 (Human Caucasian colon adenocarcinoma)

  • The anticancer effect of drNK cells is best at high doses (1.5X0^7 cells).

    The anticancer effect of drNK cells is best at high doses (1.5X0^7 cells).

  • Anticancer Effect of AiNK (1.5X107cells, day1, day4, 2 times, IV injection) : Follow-up for 28 days

    Anticancer Effect of AiNK (1.5X107cells, day1, day4, 2 times, IV injection) : Follow-up for 28 days

  • Kim HS et al., Nature Biomedical Engineering 2021 Aug 2

    Kim HS et al., Nature Biomedical Engineering 2021 Aug 2